- ￥666.90 - 4858.90
- 阿拉丁
- 上海
- C128677
- 2026年04月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存
- 英文名：
Clostripain from Clostridium histolyticum
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
C128677-1mg/C128677-10mg
| 规格： | C128677-1mg | 产品价格： | ￥666.9 |
|---|---|---|---|
| 规格： | C128677-10mg | 产品价格： | ￥4858.9 |
Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.A protease that cleaves proteins on the carboxyl bond of arginine
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文献和实验High Molecular Weight Yeast Liquid DNA Preparation
temperature in a water bath. Make a crude sucrose gradient by first adding 11 ml of 20% sucrose, then 11 ml of 15% sucrose, then carefully underlaying 3 ml of 50% sucrose in a 36 ml Beckman centrifuge tube (Ultra-Clear 25x89 mm). Slowly pour
E.Z.N.A.TM Plant DNA Maxi Protocol For Dry Specimens
for 3 min. Discard the floe through and reuse the 50 ml collection tube for set 13. 13. Centrifuge empty column 20 min at 4000x g to dry. This step is critical for removing residual ethanol that may interfere with downstream applications. Note
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
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