- ￥799.90
- 阿拉丁
- 上海
- G501304
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C储存
- 英文名：
Goat Anti-Human C9
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
G501304-1ml
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文献和实验Bradford Protein Concentration Assay
Background The Bradford protein assay (1) is one of several simple methods commonly used to determine the total protein concentration of a sample. The method is based on the proportional binding of the dye Coomassie to proteins. Within the linear range
-- Total Volume 10mL, 0.3g dry milk (3%), 100ul 10% Triton X-100 (0.1%), 100ul 100mM CaCl2 (1mM), 500ul 1M Tris-Cl pH 7.5 (50mM). Fixative -- approximately 2ml per coverslip - 37% formaldehyde diluted 1:10 with PBS (150mM NaCl, 10mM NaPi, pH 7.4)
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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