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- 文献和实验
- 技术资料
- 免疫原:
Fusion protein of human GNB1 (AA 100-340).
- 亚型:
Rabbit IgG
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
0.2 mg/mL
- 规格:
Ab105938-100μl/Ab105938-1ml/Ab105938-50μl/Ab105938-10μl
| 规格: | Ab105938-100μl | 产品价格: | ¥1574.90 |
|---|---|---|---|
| 规格: | Ab105938-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab105938-50μl | 产品价格: | ¥974.90 |
| 规格: | Ab105938-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:GNB1 Antibody
规格或纯度:ExactAb™, Validated, Carrier Free, 0.2 mg/mL
应用:WB, IF/ICC, IHC
特异性:GNB1
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:Fusion protein of human GNB1 (AA 100-340).
阳性对照:WB: Hep G2, A549, HEK293, Jurkat, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 40% Glycerol, 0.05% Sodium azide, pH 7.4
防腐剂:0.05% Sodium azide
浓度:0.2 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片
>
GNB1 Antibody (Ab105938) - Western Blot
All lanes: GNB1 Antibody (Ab105938) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 60.0 s
>
GNB1 Antibody (Ab105938) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GNB1 Antibody (Ab105938). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
>
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文献和实验) corresponds to about 50µg/ml of chromatin DNA. So the yield of chromatin (in µg) is given by A260 x dilution factor x volume x 50; Centrifuge sample (800 x g for 10 minutes at 4°C) and resuspend pellet to a chromatin DNA concentration of 0.5mg/ml
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
chamber (see note 3); Wash at least 3 times in 1X PBS for 5 minutes each; DNA counterstaining (2 minutes in 1X PBS containing 0.2mg/ml DAPI); Wash twice in 1X PBS; Mount coverslip on a slide in glycerol based mounting medium
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