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- Leading Biology
- 美国
- AMHE00022L
- 2025年07月16日
- Functional Assays
- HEK293
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 宿主：
HEK293
- 应用范围：
Functional Assays
- 规格：
100 ug
宿主：HEK293
种属反应性：Human
免疫原：Human peripheral blood lymphocytes.
靶点：hCD3
偶联物：Unconjugated
纯化方法：Affinity purification
通用名：CD3E
总结：The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
形式：Liquid
储存条件：Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
储存溶液：PBS, pH7.2
应用：Functional Assays
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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