ATP-PEG8-Biotin，阿拉丁

Crystallization of Kinesin Family Motor Proteins

) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w

Introduction to the EMSA (Gel Shift) Technique

of DNA, requiring the DNA to be labeled before performing the experiment. Traditionally, DNA is radiolabeled with 32 P by incorporating an [a -32P]dNTP during a 3´ fill-in reaction using Klenow fragment or by 5´ end labeling using [g -32P]ATP and T

User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags

composed of VHHs and suitable tags. According to the applications in which the antibodies will be applied, molecules such as fluorescent probes, biotin, and PEG can be either covalently or non-covalently linked to the antibodies. Within this chapter