Kushenol E，99119-72-9，≥96%，阿拉丁

PCR基础知识[PCR basics]

for synthesising another molecule, e.g. a single-stranded DNA molecule can be used as a template to synthesise the complementary nucleotide strand.]strands, and double strands are formed again between primers and complementary sequences[Complementary sequence

xCELLigence系统实时检测神经毒性

neurons，PCNs），并将其接种于PEI-包被的 E-Plate 96 孔板上，每孔接种的细胞密度不同，接种密度范围为 8000 至 32000 个细胞/孔。如图 3A 所示，PEI 包被不会干扰细胞阻抗的测定。在添加有2% B27 的神经基础培养基中培养 72 小时，原代神经元细胞对细胞培养的条件适应良好。xCELLigence 系统持续监测表明，可能是培养最初的几小时内，细胞贴壁、贴附于培养孔底部，CI 值在培养初期有一定升高。不同细胞密度孔中，通过 PCNs 微管相关

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

a proofreading polymerase and buffers included in the kit Triple Master Mix (Eppendorf, Hamburg, Germany). The standard reaction conditions were 96°C for 3 min, followed by 30 cycles with 96°C, 45 s, 57°C 45 s, 72°C, 1�3 min, depending on the size of the product