Cortisone Acetate，50-04-4，10mM

Northern Blot: Glyoxal/DMSO

the gel: dissolve 0.8g agarose in 100mL of 10mM NaPO4 pH 7.0 (5mL of 0.2M NaP04 pH 7.0 + 95mL "DEPC" H2 O). Make sure gel box and comb are RNase-free by treatment with 3% H2 O2 or commericial RNase removal product (e.g. RNase Zap from Ambion).

Filter Binding Assay for EBNA-1

staining by UV.Quantify percent of each band present by comparison to input DNA fragment used.(Optional) Quantify protein in each fraction by SDS-PAGE/Western.Binding Buffer : 10mM HEPES 10mM MgCl2 150mM NaCl Add 5% DMSO fresh Wash

Cell Culture Media and Solutions

with a 0.22 � cellulose acetate filter. Do NOT filter DMSO, it will dissolve the cellulose acetate membrane.   Growth media: (600 ml)   90.0 ml FBS 6.0 ml 200 mM (100X) L-glutamine 0.6 ml 50 mg/ml gentamicin reagent Note: Filter