- ￥14831.90
- 阿拉丁
- 上海
- T273098
- 2025年07月13日
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- 详细信息
- 技术资料
- 保存条件：
避光;-20°C储存;干燥
- 英文名：
6-TAMRA CPG *1000 Å*
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
T273098-1g
The light-absorbing properties of TAMRA, and spectral overlap with several commonly used fluorophores - including FAM, HEX, TET and JOE, make it useful as a FRET acceptor for the dual labeled FRET probes such as Molecular Beacons. TAMRA has been used extensively for real-time PCR and other molecular diagnostic applications. Oligonucleotides can be labeled with TAMRA using two distinct methodologies. Under standard deprotection conditions, TAMRA is not sufficiently stable; the molecule degrades in the presence of ammonium hydroxide. If standard deprotection is required, the oligonucleotide is normally synthesized with an amino group at either the 3'-, or 5'-end and post-labeled with TAMRA succinimidyl ester. Oligonucleotides synthesized using UltraMILD monomers can also be labeled directly with TAMRA, either internally by substituting any suitable dT residue with TAMRA-dT-CE phosphoramidite, or at the 3'-end using 3'-TAMRA CPG support. Subsequent deprotection of the oligo using potassium carbonate in methanol adequately removes the base protecting groups, leaving the TAMRA intact. Alternatively the deprotection of 1:1:2 t-butylamine/methanol/water allows the use of regular monomers.
Ex(nm):552
Em(nm):578
Solvent:MeCN
Chemical Structure:
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