碳酸钠/草酸浓缩液，Na2CO3 360mM and H2C

高效离子色谱柱研究

Dionex Acclaim 120 C18 5 m 120 Å (4.6 x 100 mm, ID) 涂覆柱的常规阴离子分离 Conditions:  column:  Dionex Acclaim 120 C18 5 m 120 Å (4.6 x 100 mm, ID) coated first with 5 mM Triton X-100 and then 5 mM CPC; eluent: 10 mM Na2CO3; flow rate, 1.0 ml/min

Digoxigenin-UTP RNA labeling

1. Add the following to a sterile microfuge tube on ice: -1 ug linearized plasmid DNA. -2 ul Dig RNA Labeling Mix, 10X concentrate -2 ul 10X transcription buffer -1-2 ul RNase inhibitor (DNase free) -add DEPC treated H2O up to 18 ul -2 ul RNA

Digoxigenin-UTP RNA labeling

for 15 minutes. 10X NTP Labeling Mix: 10mM ATP, CTP, GTP, 6.5mM UTP, 3.5mM DIG in Tris-HCl, pH 7.5. 10X Transcription Buffer: 400mM Tris-HCl, pH 8.0; 60mM MgCl2, 100mM dithioerythritol, 20mM spermidine, 100mM NaCl, 1U/ml RNase Inhibitor