乙酰肼，1068-57-1，≥98%，阿拉丁

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

of Uracil and Cytosine Pyrimidine Bases   Materials Sodium hydroxide (NaOH), ≥98%

胃安散治疗消化性溃疡的疗效

条件者列入治疗对象：①溃疡最大径≥0.3cm；②6周内未接受过正规抗溃疡治疗；③无幽门梗阻或大出血。随机分成两组，胃安散组（A组）162例，其中 胃溃疡 （GU）52例，十二指肠溃疡（DU）110例；西咪替丁组（B组）162例，其中GU50例，DU112例。两组的年龄、性别、疼痛、溃疡持续的时间、比例等差异均无显著性。 1.2　方法 胃安散由本院自拟中药处方，制成胶囊，每粒0.25g，每次4粒，每日3次，餐前半小时口服。西咪替丁系国产胶囊，每次0.2

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

a proofreading polymerase and buffers included in the kit Triple Master Mix (Eppendorf, Hamburg, Germany). The standard reaction conditions were 96°C for 3 min, followed by 30 cycles with 96°C, 45 s, 57°C 45 s, 72°C, 1�3 min, depending on the size of the product