甲醇中敌草隆溶液，330-54-1，1000μg/mL in

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  METHOD 1: Formaldehyde preservative � 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS). Prepare as follows: Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml

2%多聚甲醛固定剂配方二则

METHOD 1: Formaldehyde preservative 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS). Prepare as follows: Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml of 1 X PBS. Heat to 70

蛋白质印迹（Western blot）实验方案

蛋白质印迹流程点击下载：蛋白质印迹实验方案溶液和试剂●裂解缓冲液这些缓冲液可在 4 ℃ 下保存数周，或者以分装形式在 -20 ℃ 下保存长达 1 年。Nonidet-P40 (NP40) 缓冲液150 mM NaCl1.0% NP40（可用 0.1% Triton X-100 替代）50 mM Tris-HCl pH 8.0蛋白酶抑制剂RIPA 缓冲液（放射免疫沉淀试验缓冲液）150 mM NaCl1.0% NP-40 或 0.1% Triton X-1000.5% 脱氧胆酸钠0.1% SDS