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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -2°C. Avoid repeat freeze-thaw cycles.
- 保质期:
6个月
- 英文名:
SDS Peptide - middle region
- 库存:
99
- 供应商:
Biorbyt
- 规格:
100 μg
产品名:SDS Peptide - middle region
货号:orb2017088
产品中文名:SDS Peptide - middle region 蛋白
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文献和实验).In the protocol below,we describe the in-gel digestion procedure,as it is routinely performed in our laboratory,for peptide mass mapping of picomole to subpicomole quantities of protein derived from coomassie- or silver stained polyacrylamide gels.First,SDS
In-gel digestion of proteins for peptide fingerprint mapping
siliconized tube (PGC scientific). Also cut out a gel piece from a protein-free region of the gel, for a parallel control digestion to identify trypsin autoproteolysis products. A small gel particle size facilitates the removal of SDS (and coomassie
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
region of interest, and 2) the length of the “bait” peptide is sufficient for recognition by the candidate reader protein(s). Histones are most heavily modified at their N- and C-terminal tails, which are relatively unstructured and jut away
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