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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -2°C. Avoid repeat freeze-thaw cycles.
- 保质期:
6个月
- 英文名:
GRAMD2B Peptide - middle region
- 库存:
99
- 供应商:
Biorbyt
- 规格:
100 μg
产品名:GRAMD2B Peptide - middle region
货号:orb1999516
产品中文名:GRAMD2B Peptide - middle region 蛋白
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文献和实验,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
In-gel digestion of proteins for peptide fingerprint mapping
(see eg ., Shevchenko et al. 1996a and b). In the protocol below, we describe the in-gel digestion procedure, as it is routinely performed in our laboratory, for peptide mass mapping of picomole to subpicomole quantities of protein derived from coomassie- or silver
Rules for antigenic peptide selection by wxscmu1.Use more than one peptide2.Use 15-20 AA peptide3.Avoid signal peptide, transmembrane region. Avoid conserved region among both orthologs and paralogs (Do BLAST).4.Use the N- and C-terminal sequences
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