- ￥3692
- Biorbyt
- 英国
- orb1998106
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Add 100ul of sterile PBS. Final peptide concentration is 1 mg/ml in PBS. For longer periods of storage, store at -2°C. Avoid repeat freeze-thaw cycles.
- 保质期：
6个月
- 英文名：
9530077C05RIK Peptide - middle region
- 库存：
99
- 供应商：
Biorbyt
- 规格：
100 μg
产品名：9530077C05RIK Peptide - middle region
货号：orb1998106
产品中文名：9530077C05RIK Peptide - middle region 蛋白
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文献和实验Two dimensional peptide mapping
(enough to cover the piece of membrane). Start the digestion by adding 10 micrograms trypsin or chymotrypsin (Stock is 1 milligram/ml in 0.05 M NH4HCO3 stored at -70°C) . Mix by pipetting up and down and incubate at 37°C for 2 hr. 5. Vortex the sample
NH4HCO3,pH 810 mM dithiotreitol in 25 mM NH4HCO355 mM iodoacetamide in 25 mM NH4HCO3Generally,trypsin is the protease of choice for peptide mass fingerprinting,because of its reliability and its substrate specificity,yielding peptides with C-terminal
In-gel digestion of proteins for peptide fingerprint mapping
of choice for peptide mass fingerprinting, because of its reliability and its substrate specificity, yielding peptides with C-terminal basic residues (Arg and Lys), which facilitates ionization and subsequent mass spectrometric sequencing.
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