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- bs-4742P
- 2025年10月16日
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| 产品编号 | bs-4742P |
| 英文名称 | Ig kappa chain C region Antibody Blocking Peptide |
| 中文名称 | 人k链恒定区封闭多肽 |
| 英文别名 | Ig kappa chain C region; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; kappa light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; IGKC_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Immunology > Immunoglobulins > Light Chain > Kappa |
| 亚细胞定位 | Cytoplasmic and Secreted. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. |
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文献和实验Expression and Isolation of Recombinant Antibody Fragments in E. coli
antibodies (MAbs), functional derivatives (antibody fragments) carrying antigen (Ag)-binding activities, and comprising at minimum the antibody variable heavy- and light-chain domains, can be readily produced in bacterial cells (1 ). The range of antibody
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
For cloning and expressing the antigen-binding variable (Fv) portion of an antibody in Escherzchia coli , vectors have been constructed that combine the two variable regions (VH and VL ) with a peptide linker (1 –3 ). The genetic
Antigen Design Sera Purification Tech Sheet
determination, protein homology studies and protein purification. While it is quite easy to generate anti-peptide antibodies, it is important to carefully consider the ultimate use for the antibody and the sequence used to ensure success. This tech sheet
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