Recombinant Non-Specific Lipid

Non-specific lipid-transfer protein, ltp142.

Tri a 14.0101 is a non-specific lipid transfer protein and a major wheat allergen. Those who are sensitive to this allergen may have baker’s asthma, a frequent occupational allergic disease caused mainly by inhalation of cereal flour, especially wheat flour. Tri a 14.0101 consists of 4 helical fragments and irregular C-terminal regions which are highly stable to heat treatment and proteolytic attack.

Recombinant Non-Specific Lipid-Transfer Protein Tri a 14 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 13kDa.

Tri a 14.0101 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.

Sf9 insect cells.

Sterile Filtered clear solution.

Tri a 14.0101 is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Greater than 80.0% as determined by SDS-PAGE.

1. Binds IgE-type human antibodies.
2. Immunodot test with positive/negative samples.

Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.

SDS

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.