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- 2025年12月05日
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文献和实验Tetrazolium (MTT) Assay for Cellular Viability and Activity
-insoluble blue formazan, that must then be dissolved for calorimetric measurement. Ethanol or propanol ( 2 ), acid-isopropanol (0.04 M HCl in propan-2-ol) ( 3 ), acid-isopropanol plus 10% Triton X-100 ( 4 ), mineral oil (unspecified), or dimethyl sulfoxide
activity. This reaction converts the yellow salts to blue-colored formazan crystals that can be dissolved in an organic solvent whose concentration can be spectrophotometrically determined (Fig. 1 ). Owing to the many advantages of the assay, it is today
Protocol for evaluating vascular permeability in wildtype
of fomiamide (in labeled glass 7ml solvent-saver type tubes) o/n at 56°C to extract the Evans Blue. The following day, the tissues were removed from the formamide and the A620 of the samples and standard curve was measured on a spectophotometer
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