Solvent Blue 35

Blue Native Gel Electrophoresis

Blue Native Gel Electrophoresis Stock solutions 49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT 3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C

Transcription, Translation of S35-Radiolabelled Protein and Binding to GST-Fused Proteins or Antibodies (IP)

reticulocyte lysate. S35 methionine is DuPont translation grade (5mCi/mmol) Heat RNA 60°C 5 min, ice and prepare reaction cocktail. The lysate can be refrozen once. Only prepare a cocktail with the amount required. For example

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder