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- 文献和实验
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- 保存条件:
2-8℃
- 供应商:
朗智(上海)生物科技有限公司
- 规格:
0.1 mg
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Construction of a Human Antibody Domain (VH) Library
human CDRs and synthetic CDRs are combined into a single-domain (VH) framework.
Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis
Human antibody single domains are a promising new class of antibody fragments. Here we describe methods for the cloning of human VH and VL genes into phage and phagemid vectors. Furthermore, we provide detailed protocols for the generation
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