PE-Cyanine7 Anti-Mouse F4/80 A

T-cell dependent antigen specific in situ staining (Pe)

if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe

Immunohistochemistry using Anti-Ganglioside Antibodies

supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab

Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction

hydrochloride (EDC) 25 µg/ml anti‐mouse IgG antibody in 10 mM sodium acetate, pH 5.0 (Biacore) 1 M ethanolamine