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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80
- 供应商:
盛祺兆生物
- 规格:
100ug
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文献和实验【转帖】Top 100 proteomics publications
Top 100 proteomics publications ----From Ion Channel Media Group:ttp://www.ionchannelmedia.com/websites.php 1. Gavin et al. (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 2. Bonetta et al. (2006) Protein
Transgenic Mouse Core Facility
1.Restrict DNA and gel purify Cut 100ug DNA, removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of Tris saturated phenol with b
1. Cut 0.5 cm of tail and mark mouse ears or toes 2. Incubate at 55°C O/N in: 500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml) 12.5 l 20% SDS 3. Add 150 l 5 M NaCl
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