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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
AZDye 555 NHS Ester-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 555 NHS酯-1 mg,AZDye 555 NHS Ester-1 mg,AZDye 555 NHS酯-1 mg,AZDye 555 NHS Ester-1 mg
AZDye 555 NHS酯-1 mg-AZDye 555 NHS Ester-1 mg
产品货号:CCT-FP-1166-1
产品规格:1mg
AZDye™ 555 NHS Ester是一种水溶性亮橙色荧光染料,激发波长为532 nm或555 nm,使用TRITC(四甲基罗丹明)滤光片组可实现可视化。抗体、肽和蛋白质的AZDye™ 555缀合物从pH 4至pH 10是pH不敏感的。蛋白质可以用高摩尔比的AZDye 555 NHS酯标记,而没有显著的自猝灭,从而产生更亮的缀合物和更灵敏的检测。AZDye™ 555偶联物非常适合检测低丰度靶标。

AZDye™ 555 NHS Ester is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AZDye 555 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AZDye™ 555 conjugates ideally suited for detection of low abundance targets.The NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.AZDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

AZDye 555 NHS酯-1 mg-AZDye 555 NHS Ester-1 mg-20°C.
点击:AZDye 555 NHS酯-1 mg-AZDye 555 NHS Ester-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
and stoichiometry for tubulin labeled with tetramethylrhodamine (TMR) NHS ester: Tubulin concentration = [(A280 - Contribution of dye to A280 ) x Dilution Factor] / Extinction coefficient of tubulin at 280 nm TMR concentration = (A555 x Dilution Factor
LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
collect supernatant.Dilute 100 to 1000 fold and read OD at 555 nm.The volume required for reaction (in ml)is calculated as:(1.4/OD555)x mg of myosin 10.Add dye to myosin,mix gently by pipeting.Incubate on ice for 2 hr in the dark.Equilibrate column
LABELING MUSCLE ACTIN WITH IATR
1.Centrifuge in a 50Ti (1 hr,40,000 rpm)or 42.2Ti (30 min,25,000 rpm)at 4℃. 2.Measure concentration and dye/protein molar ratio.Dilute 1:40 with the dialysis buffer and read the OD at 555 nm. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 43,000
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