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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C.
- 保质期：
2年
- 英文名：
6-Azide-Trehalose (6-TreAz)-5 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
6-Azide-海藻糖(6-TreAz)-5 mg，6-Azide-Trehalose (6-TreAz)-5 mg，6-Azide-海藻糖(6-TreAz)-5 mg,6-Azide-Trehalose (6-TreAz)-5 mg
6-Azide-海藻糖(6-TreAz)-5 mg-6-Azide-Trehalose (6-TreAz)-5 mg
产品货号：CCT-1472-5
产品规格：5mg
Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with 6-azide-modified trehalose (TreAz) analogues in live mycobacteria, enabling bioorthogonal ligation with alkyne-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery.
Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with 6-azide-modified trehalose (TreAz) analogues in live mycobacteria, enabling bioorthogonal ligation with alkyne-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery. In addition, the 6-Azido-Trehalose may be employed to assess the effects of various perturbations (e.g., environmental stress, antibiotic treatment, genetic manipulation) on trehalose glycolipids and their associated biosynthetic pathways. 6-Azido-Trehalose analogue is likely to be metabolized in most, if not all, mycobacterial species given the highly conserved nature of the involved biosynthetic machinery. Importantly, the absence of trehalose metabolism in mammals invites the application of this chemical tool to investigate the trehalome during mycobacterial infection in host cells and model organisms.
6-Azide-海藻糖(6-TreAz)-5 mg-6-Azide-Trehalose (6-TreAz)-5 mg-20°C.
点击：6-Azide-海藻糖(6-TreAz)-5 mg-6-Azide-Trehalose (6-TreAz)-5 mg查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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