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- 美国
- CCT-1472-100
- 2025年07月11日
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- 保存条件：
-20°C.
- 保质期：
2年
- 英文名：
6-Azide-Trehalose (6-TreAz)-100 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
6-Azide-海藻糖(6-TreAz)-100 mg，6-Azide-Trehalose (6-TreAz)-100 mg，6-Azide-海藻糖(6-TreAz)-100 mg,6-Azide-Trehalose (6-TreAz)-100 mg
6-Azide-海藻糖(6-TreAz)-100 mg-6-Azide-Trehalose (6-TreAz)-100 mg
产品货号：CCT-1472-100
产品规格：100mg
Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with 6-azide-modified trehalose (TreAz) analogues in live mycobacteria, enabling bioorthogonal ligation with alkyne-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery.
Metabolic labeling with unnatural sugar substrates is a powerful alternative for investigating glycoconjugates in living organisms. Trehalose glycolipids can be metabolically labeled with 6-azide-modified trehalose (TreAz) analogues in live mycobacteria, enabling bioorthogonal ligation with alkyne-functionalized fluorescent probes. This strategy can be used for imaging glycolipid distribution, trafficking, and dynamics as well as metabolite profiling and discovery. In addition, the 6-Azido-Trehalose may be employed to assess the effects of various perturbations (e.g., environmental stress, antibiotic treatment, genetic manipulation) on trehalose glycolipids and their associated biosynthetic pathways. 6-Azido-Trehalose analogue is likely to be metabolized in most, if not all, mycobacterial species given the highly conserved nature of the involved biosynthetic machinery. Importantly, the absence of trehalose metabolism in mammals invites the application of this chemical tool to investigate the trehalome during mycobacterial infection in host cells and model organisms.
6-Azide-海藻糖(6-TreAz)-100 mg-6-Azide-Trehalose (6-TreAz)-100 mg-20°C.
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文献和实验Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
Acetylation (or Succinylation) of Amino Groups on Proteins
to determine the importance of amino groups to a protein's function. MATERIALS: Purified protein at approximately 0.25mg/ml (diluted in 50mM phosphate buffer pH 6.8, 3mM Na azide, and, for membrane proteins, 0.1% SDS).
Flow cytometric determination of leukocyte surface antigens in whole blood
Calibrate the flow cytometer Make the measurements Reagents PBS (Phosphate buffered saline) pH 7.4 Saponin (Sigma) - make 10 mg/ml saponin in PBS. Add azide & store in refrigerator. Blood Antibody Blood collection Collect blood
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