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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 568 Alkyne-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 568 Alkyne-25mg,AZDye 568 Alkyne-25 mg,AZDye 568 Alkyne-25mg,AZDye 568 Alkyne-25 mg
AZDye 568 Alkyne-25mg-AZDye 568 Alkyne-25 mg
产品货号:CCT-1293-25
产品规格:25mg
AZDye™ 568 Alkyne是一种水溶性、明亮的红色荧光染料,其激发非常适合568 nm激光线。AZDye™ 568炔通过铜催化的点击反应(CuAAC)与叠氮化物反应以形成稳定的三唑连接基。用于无铜叠氮化物检测的探针(AZDye™ 568 DBCO)也可用于不允许存在铜的应用。

AZDye™ 568 Alkyne is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 568 Dye and Alexa Fluor® 568.For application where the presence of copper is not acceptable, please consider our AZDye™ 568 DBCO probes for copper-less detection of azide-modified molecules.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AZDye 568 Alkyne-25mg-AZDye 568 Alkyne-25 mg-20°C. Desiccate
点击:AZDye 568 Alkyne-25mg-AZDye 568 Alkyne-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验液裂解细胞,用移液器适当吹打使细胞脱落,大瓶贴壁细胞可以先用胰酶洗脱下来后再裂解,裂解方法仿照悬浮细胞处理。 B、 悬浮细胞的处理:先将悬浮细胞置于 EP 管中 3000 rpm 离心 5 min 去掉上清培养基,每 20 μL 体系细胞加 100 μL 裂解液反复吹打至细胞完全破碎。 处理好的样品 12000 rpm 离心 5 min,取上清即为提取的蛋白。置于 4 ℃ 可保存一周,-20℃ 可保存约 2 个月,-80 ℃ 可保存半年。 蛋白浓度的测定:酶标板,酶标仪、离心机、1 mg/mL
Ribonuclease A (RNase A), DNase-free
The absence of endodeoxyribonucleases, exodeoxyribonucleases and proteases confirmed by appropriate quality tests. Functionally tested for RNA digestion in a plasmid DNA purification procedure.Concentration10 mg/ml.Protein concentration is determined
在101kPa大气压和25℃下,把1mol气态的AB分子分离成气态的A和B原子要吸收的能量(kJ/mol),叫做键能。键能也是两种气体A和B结合生成1mol气态分子AB时放出来的能量。键能是表征共价键强弱的物理量,常用E(A—B)表示。E越大,A—B键越牢,AB分子越稳定。键能数据一般从测定分子离解能(D)来求得。双原子分子的键能和离解能在数值上是相同的。例如H—F(气)=H(气)+F(气),这一过程中的E(H—F)=D(H—F)=568kJ/mol。原子分子的键能和离解能在概念
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