Agilent安捷伦 5191-4293ValueLab f

ChIP-Chip E. coli

150 mM NaCl 15ml 5M 1 mM EDTA 1ml 0.5M 1% Triton X 100 5ml 0.1 % Sodium deoxycholate 0.5g 0.1 % SDS 5ml 10%  

cDNA/AFLP Protocol

in magnetic stand for 30 sec or longer to collect the beads. c) remove supernatent and wash 3X with 200 ul of 0.5X SSC d) finally resuspend the SA-PMPs in 100ul of 0.5X SSC for each 200 ul of beads originally removed - each 100 ul aliquot

【交流】染色体免疫沉淀技术详细步骤-young lab ！！！

50 mM  1M Hepes-KOH, pH 7.5  5.0 ml 140 mM  5M NaCl  2.8 ml 1 mM  0.5M EDTA  0.2 ml 10%  50% glycerol  20.0 ml 0.5%  10% NP-40  5.0 ml 0.25%  10% Triton X-100  2.5 ml   ddH2O  64.5 ml Lysis Buffer 2 (LB2) Final Conc.  Stock  For 100 ml 10