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文献和实验RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
96 Well Mating Protocol platewise
% Glycerol (sterile) 10 ml 20% L-arabinose (filter sterile) 1 ml Carbenicillin at 100mg/ml (for pMSCV selection) AND antibiotic to select for your insert POUR YEGlu: 1 liter MilliQ water 5 g yeast extract 10 g NaCl autoclave and cool add 8 ml of 50
96 Well Mating Protocol platewise
(filter sterile)1 ml Carbenicillin at 100mg/ml (for pMSCV selection)AND antibiotic to select for your insertPOUR YEGlu:1 liter MilliQ water5 g yeast extract10 g NaClautoclave and cool add 8 ml of 50% Glucose (filter sterile) Strain and vector information
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