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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
三年
- 英文名:
SLC12A1 Human bumetanide-sensitive Na-K-2Cl cotransporter (NKCC2) mRNA.
- 库存:
有
- 供应商:
GeneCopoeia
- 规格:
10μg
NCBI推荐的基因克隆供应商
产品优势
多——140,000+个ORF克隆;涵盖20,000+个人类基因;200+种哺乳动物、 慢病毒等表达载体;50+种 蛋白融合标签
快—— 可直接用于蛋白表达,无需亚克隆步骤
好—— 保证序列,经过全长测序
省—— 价格优惠
Next-Day 克隆
多——涵盖全基因组
快——今天订货,明天发货
好——DNA 序列保证,用户信赖 --- 几千篇期刊发表论文
省——价格低至 199元


客户发表文章举例
◆ Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3.2019. Cell, IF 31.398. The human cDNA library constructs coding ~5600 full-length plasma membrane protein and ~1000 secreted protein were purchased from GeneCopoeia.
◆ Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage. 2020. Cell, IF 30.17. OMICSLINK™ hPiezo1 ORF expression clone
◆ Identification of recurrent FHL2-GLI2 oncogenic fusion in sclerosing stromal tumors of the ovary. 2020. Nature Communications, IF 12.30. OMICSLINK™ wild type FHL2 , wild type and mutant GLI2, FHL2-GLI2 fusion ORF expression clones.
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文献和实验[1] Sanmarco, L.M., Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature, 2023. doi: 10.1038/s41586-023-06409-6
[2] Soto, J.S., et al. Astrocyte–neuron subproteomes and ob sessive–compulsive disorder mechanisms. Nature, 2023. doi: 10.1038/s41586-023-05927-7
[3] Turrell, F.K., et al. Age-associated microenvironmental changes highlight the role of PDGF-C in ER+ breast cancer metastatic relapse. Nature Cancer, 2023. doi: 10.1038/s43018-023-00525-y
[4] Wang, Y., et al. DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC. Nature Communications, 2023. doi: 10.1038/s41467-023-38160-x[5] Zheng, L., et al., The CD8α-PILRα interaction maintains CD8+ T cell quiescence. Science, 2022. doi: 10.1126/science.aaz8674
Nature:揭秘常用降压药作用机制,冯亮团队破解关键转运蛋白结构
资料: [1] Fan, M., Zhang, J., Lee, CL. et al. Structure and thiazide inhibition mechanism of the human Na–Cl cotransporter. Nature (2023). https://doi.org/10.1038/s41586-023-05718-0
cDNA Expression Cloning in Mammalian Cells
., Blakely, R.D., and Amara, S.G. 1991. Expression cloning of a cocaine‐ and antidepressant‐sensitive human noradrenaline transporter. Nature 350:350‐354. Seeburg, P.H., Wisden, W
扩增,从而使mRNA的PCR步骤更为简化,所需样品量减少到最低限度,临床小样品的检测非常有利。用一步法扩增可检测出总RNA中小于1ng的低丰度mRNA。该法还可用于低丰度mRNA的cDNA文库的构建及特异cD-NA的克隆,并有可能与Taq酶的测序技术相组合,使得自动反转录、基因扩增与基因转录产物的测序在一个试管中进行。 最近Cetus公司推出rTth逆转录酶,兼具有DNA多聚酶的活性,对于RNA的发展起了很大的推动作用。有关rTth逆转录酶法参考第四节 有关内容。 十一、定量PCR
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