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- GC-Mm32682
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
三年
- 英文名：
Mus musculus unc-79 homolog (C. elegans) (Unc79), mRNA.
- 库存：
有
- 供应商：
GeneCopoeia
- 规格：
10μg
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客户发表文章举例
◆ Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3.2019. Cell, IF 31.398. The human cDNA library constructs coding ~5600 full-length plasma membrane protein and ~1000 secreted protein were purchased from GeneCopoeia.
◆ Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage. 2020. Cell, IF 30.17. OMICSLINK™ hPiezo1 ORF expression clone
◆ Identification of recurrent FHL2-GLI2 oncogenic fusion in sclerosing stromal tumors of the ovary. 2020. Nature Communications, IF 12.30. OMICSLINK™ wild type FHL2 , wild type and mutant GLI2, FHL2-GLI2 fusion ORF expression clones.
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文献和实验[1] Sanmarco, L.M., Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature, 2023. doi: 10.1038/s41586-023-06409-6
[2] Soto, J.S., et al. Astrocyte–neuron subproteomes and ob sessive–compulsive disorder mechanisms. Nature, 2023. doi: 10.1038/s41586-023-05927-7
[3] Turrell, F.K., et al. Age-associated microenvironmental changes highlight the role of PDGF-C in ER+ breast cancer metastatic relapse. Nature Cancer, 2023. doi: 10.1038/s43018-023-00525-y
[4] Wang, Y., et al. DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC. Nature Communications, 2023. doi: 10.1038/s41467-023-38160-x[5] Zheng, L., et al., The CD8α-PILRα interaction maintains CD8+ T cell quiescence. Science, 2022. doi: 10.1126/science.aaz9741
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos Geraldine Seydoux and Andrew Fire Adapted from Seydoux, G. and Fire, A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans
Fire和Timmons 继续实验。他们将一种能够表达与+ C. elegans unc-22基因同源的双链RNA的基因工程 细菌 喂食线虫，结果线虫表现出类似unc-22缺陷的表型。后继的实验表明将线虫浸入双链RNA中同样可以诱导基因沉默——这种技术使得大规模筛选线虫R NA i 诱导的功能缺失突变 体成为可能，并引发后继的大量针对这种模式生物 基因敲除的研究。 在随后的短短一年内，RNAi现象又陆续在果蝇 ，锥体虫，真菌，涡虫，植物 及斑马鱼 等真核生物的研究中被发现，这种情况揭示
of RNAi inspired Fire and Timmons to try feeding nematodes bacteria that had been engineered to express dsRNA homologous to the C. elegans unc-22 gene. Surprisingly, these worms developed an unc-22 null-like phenotype (11-13). Further work showed
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