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- GeneCopoeia
- 广州
- GC-Rn21473
- 2025年07月15日
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Arxes1 Mus musculus adipocyte-related X-chromosome expressed sequence 1 (Arxes1), mRNA.
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Gdpd1 Rattus norvegicus glycerophosphodiester phosphodiesterase domain containing 1 (Gdpd1), mRNA.
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RHD Homo sapiens Rh blood group D antigen (RHD), transcript variant 1, mRNA.
询价GGT6 Homo sapiens gamma-glutamyltransferase 6 (GGT6), transcript variant 5, mRNA.
询价Pon1 Mus musculus paraoxonase 1 (Pon1), mRNA.
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
三年
- 英文名：
Sp6 Rattus norvegicus Sp6 transcription factor (Sp6), mRNA.
- 库存：
有
- 供应商：
GeneCopoeia
- 规格：
10μg
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客户发表文章举例
◆ Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3.2019. Cell, IF 31.398. The human cDNA library constructs coding ~5600 full-length plasma membrane protein and ~1000 secreted protein were purchased from GeneCopoeia.
◆ Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage. 2020. Cell, IF 30.17. OMICSLINK™ hPiezo1 ORF expression clone
◆ Identification of recurrent FHL2-GLI2 oncogenic fusion in sclerosing stromal tumors of the ovary. 2020. Nature Communications, IF 12.30. OMICSLINK™ wild type FHL2 , wild type and mutant GLI2, FHL2-GLI2 fusion ORF expression clones.
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文献和实验In Vitro Transcription and Translation
Large amounts of active mRNA can be synthesized in vitro. In vitro-transcribed RNA molecules may subsequently be used for various purposes, e.g., in vitro translation. Several plasmids are available that are used to produce transcript molecules
Quantification of mRNA Levels Using Ribonuclease Protection Assay
of neurotrophin mRNA. This DNA fragment can either be part of a cDNA clone or a genomic subfragment, but it should be selected so that the plasmid construct can be digested with a specific restriction enzyme prior to transcription, to yield a labeled RNA
How do you synthesize your dsRNA
7 & SP6 or T7 & T3 promoter primers depending on the plasmid. Check the PCR results to ensure that you have a band of the expected size. We use the Ambion MEGAscript T7, T3, & SP6 kits (Cat.# : 1334, 1338, 1330) for the transcription reaction. With T
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