- 询价
- Charles River
- 美国
- COV-PBMC-1144
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
盛祺兆生物
- 细胞类型：
Mononuclear Cells
- 细胞形态：
冻存
- 运输方式：
液氮
- 规格：
5-10M cells
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文献和实验Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
实验原理 A mixture of biotinylated monoclonal antibodies against the non-CD8 T cells is added to the starting sample and allowed to bind to the cells. Depletion MyOne™ SA Dynabeads® are added and will bind to the antibody
Isolation of Untouched Human CD4+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
buffer. 3. Isolation Procedure This protocol is based on 1 × 107 PBMC and is scalable from 1 × 107 - 5 × 108 cells according to. Volumes for human CD4 T cell isolation (scalable from 1 × 107 to 5 × 108 PBMC). For lower cell numbers
peripheral blood mononuclear cells (PBMC). The assay assumes a direct relationship between expressed p24 antigen and the number of target PBMC infected. Therefore, the optimal time of p24 antigen measurement may vary due to different growth kinetics
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