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- Elabscience
- E-KAB-0092
- 湖北武汉
- 2025年11月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 检测方法：
ELISA双抗体夹心法
- 适应物种：
Mouse
- 标记物：
Biotin
- 规格：
50μL&100μg
Mouse MMP-9 Antibody Pair Set抗体对
产品名称：Mouse MMP-9 Antibody Pair Set
货号：E-KAB-0092
| Background | Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions,such as morphogenesis,differentiation,angiogenesis,and tissue remodeling,as well as pathological processes including inflammation,arthritis,cardiovascular diseases,pulmonary diseases,and tumor invasion. MMP9,also known as 92-kDa gelatinase B/type IV collagenase,is secreted from neutrophils,macrophages,and some transformed cells,and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodeling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils and is particularly important in the pathogenesis of inflammatory,infectious,and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines,or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent on the delicate balance between MMPs and their inhibitors,excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence,regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. Besides,the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention. |
| Synonyms | MMP9; CLG4B,Gelatinase B; GELB; MANDP2; 92kDa Type IV Collagenase; 92 KDa Gelatinase |
| Swissprot | P41245 |
| Reactivity | Mouse |
| Specificity | Detects Mouse MMP-9 in ELISAs |
| Buffer | Capture Antibody:PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4; Detection Antibody:PBS with 0.04% Proclin 300, 1% protective protein, 50% glycerol, pH 7.4 |
| Storage | -20℃ |
| Expiration date | 12个月 |
Elabscience®抗体对简介
Elabscience®抗体对主要应用于ELISA双抗体夹心法，其组分包括捕获抗体和检测抗体。捕获 抗体一般是未标记的单抗或多抗，而检测抗体是已经标记好了的生物素的单抗或多抗。 Elabscience®提供300+种抗体对产品，具有高浓度、高特异性，便于客户创建自己的ELISA 体系，经济实惠。
Mouse MMP-9 Antibody Pair Set抗体对Elabscience®提供多种高品质的ELISA试剂盒，产品覆盖人、 小鼠、 大鼠、 兔、 猴、 通用等反应性物种。我们的ELISA产品体系稳定，线性范围好，特异性和灵敏度高,热销全球100多个国家，产品获得ISO9001认证及欧盟CE认证，并拥有超21,818篇文献引用，深得广大科研使用者的一致好评！
Mouse MMP-9 Antibody Pair Set抗体对-20℃保存12个月
Mouse MMP-9 Antibody Pair Set抗体对产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Identification of a Mutant Kinase/ATP Analog Pair
(if [y-32 P]ATP analog is used) or immunoblotted with a phosphospecific antibody (if available) to determine the extent of substrate phosphorylation. The kinase/ATP analog pair that provides the best phosphorylation of the substrate is then used
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
You will need: 13.5 day pregnant mouse (we use MTK NEO inbred white mice) 2 sets sterile instruments one containing a pair of curved forceps and a pair of iris scissors one containing two pairs curved forceps, one pair iris
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