- 询价
- BPS Bioscience
- 79704-2
- 美国
- 2025年07月12日
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- 文献和实验
- 技术资料
- 库存：
Media & Reagents
- 供应商：
盛祺兆生物
- 规格：
500 ml
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文献和实验ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS
-abundance proteins (like Myc!).The protocol is described for harvesting mammalian cells from 6 or 10 cm tissue-culture dishes.A.CELL HARVEST.1.Remove media from cells.Wash in ice-cold PBS.2.Scrape cells in 1 ml of ice-cold PBS.Transfer to 1.5 ml microfuge
MEDIUM FOR EMBRYO RESCUE (FOR 1 L)
Put 500 mL of distilled water in a 1-L beaker. Add the following: Inorganic salts Muraschige�Skoog Organic medium � 34.6 g NaH2PO4H2O (stock 17 g/L) � 10-mL stock
Yeast Extract for Friis Medium
1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.2.Place in incubator at 37°C for 20 minutes.3.Heat to 90-100°C for 5 minutes.4.Cool on ice.5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C).6.Filter supernatant
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