- ￥4437
- BPS Bioscience
- 美国
- 71313-2
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Protein
- 供应商：
盛祺兆生物
- 规格：
50 µg
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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
, 50 µg streptomycin and 50 µM 2-ME in the absence or presence of HAT or HT for selection (I-20). Procedure: NOTE: All washes are at 1,000 rpm for 10 minutes at 4° C using serum free media Tissue Collection: 1. Sacrifice animal
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