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- 详细信息
- 文献和实验
- 技术资料
- 库存:
Cell Pool
- 供应商:
盛祺兆生物
- 规格:
1 vial
The Cas9 Inducible (Tet-On) iPS Cell Pool expresses the Cas9 (Streptococcus pyogenes CRISPR associated protein 9) gene under the control of a tight TRE tetracycline-inducible promoter, introduced into PBMC-derived human iPS cells via lentiviral transduction [Cas9 Lentivirus (inducible TET on), BPS Bioscience #78794]. Cas9 expression can be induced with doxycycline treatment, allowing temporal control of its expression. Cas9 Inducible (Tet-On) iPS cells can be transduced with single-guide RNA (sgRNA) targeting gene(s) of interest to generate genetically engineered cell pools or cell lines.
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文献和实验相关实验
. Li, Z.J. Chen, H. Yang, Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells, Dev Cell 45(4) (2018) 526-536 e5. [4] P.D. Hsu, E.S. Lander, F. Zhang, Development and applications of CRISPR-Cas9 for genome engineering, Cell
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Cas9 Inducible (Tet-On) iPS Cell Pool1 vial
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