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- 详细信息
- 文献和实验
- 技术资料
- 库存:
Assay Kit
- 供应商:
盛祺兆生物
- 规格:
384 rxns.
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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
In Vitro Transcription Assay (Run-off Assay) using Permeabil
. Preparation of Slot Blot Membrane for Hybridization (see Hint #4). 1. Add 1 volume of 0.5 N NaOH to the target DNA (see Hint #5). Two μg of DNA are needed for each slot of the slot blot 2. Incubate for 15 min at 85℃ to denature the DNA . 3. Incubate on ice
A Fluorescence-Based High-Throughput Screening Assay to Identify HIV-1 Inhibitors
T lymphoblastoid MT4 cell line, stably transfected with a construct carrying an HIV-1 long terminal repeat promoter driving the expression of a reporter gene (enhanced green fluorescent protein). This assay runs in a 384-well format and enables
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