- ￥12240
- BPS Bioscience
- 美国
- 78217-2
- 2026年04月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Assay Kit
- 供应商：
盛祺兆生物
- 规格：
384 reactions
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文献和实验has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity in small,snap frozen pieces of tissue (e.g.,colon polyps),or in cultured cells. 1.Homogenize Cells/Tissues For cells in culture: Harvest
Quantitate proteins in 15 ml aliquots of lysates (in duplicate) by using a Bradford assay (I have been using the Biorad kit), using BSA as standard. Don't forget to add 15 ml LB to each BSA sample and to the blank tube. Use the same LB batch as that used
Enzyme Kinetics assay of the WT
Enzyme Kinetics assay of the WT To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc., Lavellette, NJ), frozen on dry ice and stored
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