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- 详细信息
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- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
天根生化科技(北京)有限公司
- 规格:
3000U
| 目录号 | 规格 | 目录价 |
| RT410-02 | 3000U | 780元 |
10U/μl
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文献和实验[1] Lin C, Bonsu A A F K, Li J, et al. Application of metagenomic next-generation sequencing for suspected infected pancreatic necrosis[J]. Pancreatology, 2022, 22(7): 864-870.
[2] Ochoa-Viñals N, Alonso-Estrada D, Faife-Pérez E, et al. β-Carotene production from sugarcane molasses by a newly isolated Rhodotorula toruloides L/24-26-1[J]. Archives of Microbiology, 2024, 206(6): 245.
[3] Li B, He Q, Rui Y, et al. Rapid detection for infected ascites in cirrhosis using metagenome next‐generation sequencing: A case series[J]. Liver International, 2022, 42(1): 173-179.
[4] Liu K, Liu S, Ran C. The effect of air-water-plasma-jet-activated water on Penicillium: the reaction of HNO2 and H2O2 under acidic condition[J]. Frontiers in Physics, 2020, 8: 242.
。(8) 溶壁酶酶液:1.5%,用0.6MMgSO4配制,过滤除菌;(9) 培养皿:根据样品个数2. 制备方法:(1) 称取离心管重量并记录;(2) 用接种针挑出菌丝,并用灭菌双蒸水冲洗一次,然后用无菌的0.6M MgSO4冲洗两次,用灭菌的滤纸吸干,放入已知重量的离心管,称重并记录;(3) 计算得到菌丝重量,按0.2(g):1(mL)加入灭菌酶液,震荡均匀;(4) 28℃,3~4小时,摇床震荡(轻微),酶解,同时每30min镜检观察原生质体数量;(5) 灭菌注射器(含脱脂棉和玻璃钎维)过滤
is 20-35 g cells. Anything less than 18g will give poor extracts. If cells are overgrown, lyticase will work poorly in spheroplasting cells. Resuspend cell pellets in 35 ml 50 mM Tris 7.5, 30 mM DTT. Usually this can be done by gently shaking
the SDS and lyticase solutions, CHROMA SPIN-1000 DEPC-H2O Columns, and 2-ml centrifuge tubes for use with the columns.• Appropriate SD liquid or agar medium to keep selection on the plasmids (Appendix C.A;Appendix E).• Sterile, 1.5-ml microcentrifuge tubes
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