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文献和实验Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion
The plasticity of conducting airway epithelia is well recognized (1 –3 ). Under normal conditions, the epithelia express mucociliary function, which is the first pulmonary defense mechanism against inhaled air pollutants. Aberrance
Isolation and Culture of Human Alveolar Epithelial Cells
and other animals’ lungs are available (1 ), no cell lines that possess significant functional properties of alveolar epithelial cells (AEC) are reported to date (1 ). Primary culture of AEC is, therefore, used for most in vitro studies of alveolar epithelial
without splitting. Then, the cells are fed with a medium designed for the maintenance and growth of mature distal airway epithelial cells (small airway growth medium, SAGM) for 3 d. Characterization of the alveolar type II cells was done using real-time reverse
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