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文献和实验Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones
The molecular engineering of recombinant plasmid DNA clones containing the full-length and replication-competent feline foamy (retro)virus (FFV) proviral genome is described. The methods used to combine subgenomic FFV DNA fragments
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
against the protein of interest (i.e., the suspected "bridging protein" or the protein whose mediating physical proximities the investigator wishes to map). Thereafter, the cross-links are reversed, and the DNA is purified further. The fragments obtained
Two critical events are the signature of the life cycle of retroviruses (1 ). The first is reverse transcription, whereby the single-stranded RNA genome of the retrovirus is copied into double-stranded DNA. The second of these events
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