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文献和实验Long-PCR Amplification of Human Genomic DNA
Standard polymerase chain reaction (PCR) protocols amplify relatively small fragments precluding the use of this approach when examining gross rearrangements of DNA. By using combinations of DNA polymerases, which feature either good
Rapid Isolation of Genomic DNA from Human Oral Mucosa
C Add 250 μl 4.5 M NaCl and mix Pellet cellular debris (13000 rpm for 4 min). Genomic DNA stays in the supernatant Precipitate DNA by adding 650 μl of isopropanol to the supernatant and centrifuge 5 min at 13000 rpm Re-suspend
实验原理 The Dynabeads SILANE genomic DNA kit provides an excellent tool for isolation of genomic DNA from human blood, following a simple separation protocol. A buffer is first added to the sample for lysis, followed
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