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- Broadpharm
- BP-22108
- 2025年07月10日
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上海玉博生物科技有限公司
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文献和实验were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Site‐Specific Protein Labeling with SNAP‐Tags
/) EZ‐Link NHS‐PEG 12 ‐Biotin (Thermo Scientific) Phosphate‐buffered saline (PBS; appendix 2E ) Cell
Amino-allyl Reverse Transcription
Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy5 reactive pack, Amersham catalog PA 23001 and PA 25001) in 72 ul DMSO. Aliquot 16 x 4.5 ul and either use immediately or dry
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