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文献和实验Genotype 1a HCV (H77S) Infection System
research, the parallel development of a tractable genotype 1a infection system (H77S virus) has provided significant advantages in assessing genotype 1-specific interventions, given the highly heterogeneous nature of HCV. H77S RNA contains five cell culture
Phosphoamino acid analysis:Mark Kamps's method
virus phosphorylates tyrosine.Proc Natl Acad Sci U S A 1980 77:1311-1315. Kamps,M.P.,and Sefton,B.M.(1989)Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins.Anal Biochem
Preparation and use of Zamboni’s-fixed Frozen Sections in Immunohistochemistry
33.77g Na2H PO4. 7H2O. 1000ml distilled H2O). This fixative is stable at 25oC for 12 months. PROCEDURES 1. Cut 7μm thick sections of frozen tissue and fix immediately in Zamboni’s fixative for 10 minutes. 2. Wash 3 x 10 minutes in Tris
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