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文献和实验TRICINE GELS Protocol (For Low MW proteins (
Stock Solutions1) Gel Buffer: 3 M TrisHCl pH8.45 + 0.3% SDS. Keep RT. (Prepare: 18.15gr Tris + 150mg SDS + H2O up to 50ml. Adjust pH before you add the SDS) 2) 40% Acrylamide solution 29:1 (Acrylamide 29 / Methylene bis Acrylamide 1) or 40
with theoretical AA compositions of proteins in databases. For identification of proteins from 2-D gels, we match the AA composition in conjunction with estimated protein pI and Mw. Protein identification by compositional analysis is best used
实验试剂 Material Amount Storage Stability DAPI dihydrochloride (MW = 350
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