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文献和实验蛋白质纯化的原理和方法（Protein Purification Principles and Methods）
: Periplasmic and cytoplasmic proteins are released •Centrifugation leads to a soluble fraction(supernatant) which contains all soluble periplasmic and cytoplasmic proteins and a membrane fraction from which membrane bound proteins can be solubilised
Not only do membrane proteins represent a substantial fraction of the information in a genome, but also they are responsible for many essential biological functions, some of which are unique (e.g., as membrane transporters). As a result
Typical result with Fab fragment labeling (50µg used) Labeling efficiency (incorporation) should be 10-90% (depends on amount of protein added). Collect appropriate fraction (in this case, fr #3+4), add 1/4 vol of 5%BSA*, and store
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