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文献和实验TRICINE GELS Protocol (For Low MW proteins (
traces Keep in aliquots of 1ml at -20C Separating and Stacking Gel SOLUTIONS Separating gel Short gels: 4 Large gels
Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
with theoretical AA compositions of proteins in databases. For identification of proteins from 2-D gels, we match the AA composition in conjunction with estimated protein pI and Mw. Protein identification by compositional analysis is best used
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