Broadpharm代理商-Succinimidyl Car

TRICINE GELS Protocol (For Low MW proteins (

Solution   Methanol CP  500ml 50% Acetic Acid CP 100ml 10% H2O 400ml  

单组分溶液配制

. SDS Sodium dodecyl sulfate, sodium lauryl sulfate; [CH3 (CH2 )10 CH2 SO4 ]Na; Mw=288.4g/M; (store at NT). Conc. Stock %(w/w) 100 ml 250 ml 300 ml 400 ml SDS 10%(w/v) solid 9.82 10g 25g 30g 40g

Phycoerythrin conjugation protocol

concentrator (Centricon 30 or 100 are fine)   Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000