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文献和实验(1.6x-2x) might "stick" the newly synthesized strands better to the template, allowing the polymerase to finish its task. PCR reactions in which only KCl or Tris-HCl concentrations were varied, showed that the described effect is due to the salt
Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
posttranslational modifications of histones, and the cumbersome use of reverse-phase high-performance liquid chromatography. The three methodologies are shorter than the conventionally used protocols. The salt-urea method exploits the stability of the cell nucleus
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