Broadpharm代理商-TCEP, Hydrochlor

【求助】PKC抑制剂Bisindolylmaleimide区别

licanming 我在sigma上看到许多Bisindolylmaleimide，有Bisindolylmaleimide I II IV V VI VII VIII acetate salt X hydrochloride XI hydrochloride I hydrochloride等10中不同的试剂，有些说是针对大鼠脑PKC的（说明书），我不知是否指组织特异性（英文理解力有限）？但I II VI我还未看出有什么区别！文献大多选用I，但I要9000多人民

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

buffer; 1ml of ChIP high salt buffer; 1ml of ChIP wash buffer; (note 4)Pulldown (2), Flag-tag specific After the last wash elute the complex by adding 100µl of ChIP elution buffer. Incubate the samples at 65°C for 10 minutes

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

polymerase (REDTaq; Sigma D4309) (for polymerase chain reaction [PCR]; see Step 45 and Discussion) dNTP set (100 mM; Invitrogen) (for PCR; see Step 45 and Discussion) Ethanol (70% and 100%) Fetal bovine serum (10% in PBS) Formaldehyde