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- VMRD
- VMRD-SLD-IFA-FIP1
- 2025年07月11日
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上海玉博生物科技有限公司
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文献和实验Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
to depression slide and observing on 4X or by observing tube of embryos using dissecting microscope. Be careful not to get AP substrate on your hands (wear gloves) or on your microscope . Do not leave light turned on between observations. To stop the reaction
, 70% ethanol Formamide (FA), (e.g. from Merck) Master mix ( 20% dextransulfate/4xSSC) SSC (20x) 70% FA/2xSSC for chromosome denaturation (120 µl per slide) 18x18 mm2 cover slips, 60x24 mm2 cover slips (e.g. Menzel) rubber cement (Fixogum
image of a whole mount from a 4 wk old virgin Protocol Spread tissue on glass slide Fix in Carnoy's fixative for 2 to 4 hours at r.t. Wash in 70 % EtOH for 15 min Change gradually to distilled water
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